Development of genetic diagnostic methods for detection for novel coronavirus 2019(nCoV-2019) in Japan

Shirato K.,
Nao N.,
Katano H.,
Takayama I.,
Saito S.,
Kato F.,
Katoh H.,
Sakata M.,
Nakatsu Y.,
Mori Y.,
Kageyama T.,
Matsuyama S.,
Takeda M.
Document Type
Source Title
Japanese Journal of Infectious Diseases
National Institute of Health


During the emergence of novel coronavirus 2019 (nCoV) outbreak in Wuhan city, China at the end of 2019, there was movement of many airline travelers between Wuhan and Japan, suggesting that the Japanese population was at high risk of infection by the virus. Hence, we urgently developed diagnostic systems for detection of 2019 nCoV. Two nested RT-PCR and two real-time RT-PCR assays were adapted for use in Japan. As of February 8, 2020, these assays have successfully detected 25 positive cases of infection in Japan. © 2020, National Institute of Health. All rights reserved.

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Index Keywords

article; controlled study; human; Japan; nonhuman; real time reverse transcription polymerase chain reaction; Severe acute respiratory syndrome coronavirus 2; Betacoronavirus; Coronavirus infection; genetics; Japan; laboratory technique; pandemic; procedures; reverse transcription polymerase chain reaction; virus pneumonia; coronavirus spike glycoprotein; ORF1ab polyprotein, SARS-CoV-2; spike protein, SARS-CoV-2; viral protein; virus RNA; Betacoronavirus; Clinical Laboratory Techniques; Coronavirus Infections; Humans; Japan; Pandemics; Pneumonia, Viral; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral; Spike Glycoprotein, Coronavirus; Viral Proteins